Macaques co‐immunized with SIVgag/pol‐HIVenv and IL‐12 plasmid have increased cellular responses
Identifieur interne : 002C44 ( Main/Exploration ); précédent : 002C43; suivant : 002C45Macaques co‐immunized with SIVgag/pol‐HIVenv and IL‐12 plasmid have increased cellular responses
Auteurs : T. M. Robinson ; M. K. Sidhu [États-Unis] ; G. N. Pavlakis [États-Unis] ; B. K. Felber [États-Unis] ; P. Silvera [États-Unis] ; M. G. Lewis [États-Unis] ; J. Eldridge [États-Unis] ; D. B. Weiner [États-Unis] ; J. D. Boyer [États-Unis]Source :
- Journal of Medical Primatology [ 0047-2565 ] ; 2007-08.
Abstract
Background The cell mediated immune profiles following immunization with a recombinant DNA vaccine was assessed in the simian‐human immunodeficiency virus (SHIV) and Macaque model. Earlier work demonstrated increased numbers of antigen specific CD8 and CD4 effector cells able to secrete IFN‐γ. Method The vaccine strategy included co‐immunization of a DNA based vaccine alone or in combination with a macaque IL‐12 expressing plasmid (pmacIL12). Antigen activated lymphocytes were studied for activation of a set of immunological molecules. Results The current study demonstrates lymphocytes isolated and activated from the group that was immunized with DNA and pmacIL12 had a higher level of IFN‐γ producing cells. We also observed a different immunological profile when comparing the cells isolated from macaques immunized with DNA as compared to those animals that also received pmacIL12. Conclusion The observed immune profiles are reflective of the co‐delivery of pmacIL12 and demonstrates that IL‐12 can increase the magnitude and polyfunctionality of the cellular immune response.
Url:
DOI: 10.1111/j.1600-0684.2007.00245.x
Affiliations:
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Lewis</name></author><author><name sortKey="Eldridge, J" sort="Eldridge, J" uniqKey="Eldridge J" first="J." last="Eldridge">J. Eldridge</name></author><author><name sortKey="Weiner, D B" sort="Weiner, D B" uniqKey="Weiner D" first="D. B." last="Weiner">D. B. Weiner</name></author><author><name sortKey="Boyer, J D" sort="Boyer, J D" uniqKey="Boyer J" first="J. D." last="Boyer">J. D. 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M." last="Robinson">T. M. Robinson</name><affiliation><wicri:noCountry code="no comma"> Jefferson University</wicri:noCountry></affiliation></author><author><name sortKey="Sidhu, M K" sort="Sidhu, M K" uniqKey="Sidhu M" first="M. K." last="Sidhu">M. K. Sidhu</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">État de New York</region></placeName><wicri:cityArea> Vaccine Discovery, Wyeth, Pearl River</wicri:cityArea></affiliation></author><author><name sortKey="Pavlakis, G N" sort="Pavlakis, G N" uniqKey="Pavlakis G" first="G. N." last="Pavlakis">G. N. Pavlakis</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea> National Cancer Institute, Frederick</wicri:cityArea></affiliation></author><author><name sortKey="Felber, B K" sort="Felber, B K" uniqKey="Felber B" first="B. K." last="Felber">B. K. Felber</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea> National Cancer Institute, Frederick</wicri:cityArea></affiliation></author><author><name sortKey="Silvera, P" sort="Silvera, P" uniqKey="Silvera P" first="P." last="Silvera">P. Silvera</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea> SRI, Frederick</wicri:cityArea></affiliation></author><author><name sortKey="Lewis, M G" sort="Lewis, M G" uniqKey="Lewis M" first="M. G." last="Lewis">M. G. Lewis</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea> Bioqual, Rockville</wicri:cityArea></affiliation></author><author><name sortKey="Eldridge, J" sort="Eldridge, J" uniqKey="Eldridge J" first="J." last="Eldridge">J. Eldridge</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">État de New York</region></placeName><wicri:cityArea> Vaccine Discovery, Wyeth, Pearl River</wicri:cityArea></affiliation></author><author><name sortKey="Weiner, D B" sort="Weiner, D B" uniqKey="Weiner D" first="D. B." last="Weiner">D. B. Weiner</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Pennsylvanie</region></placeName><wicri:cityArea> Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine</wicri:cityArea></affiliation></author><author><name sortKey="Boyer, J D" sort="Boyer, J D" uniqKey="Boyer J" first="J. D." last="Boyer">J. D. Boyer</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Pennsylvanie</region></placeName><wicri:cityArea> Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine</wicri:cityArea></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Journal of Medical Primatology</title><title level="j" type="alt">JOURNAL OF MEDICAL PRIMATOLOGY</title><idno type="ISSN">0047-2565</idno><idno type="eISSN">1600-0684</idno><imprint><biblScope unit="vol">36</biblScope><biblScope unit="issue">4‐5</biblScope><biblScope unit="page" from="276">276</biblScope><biblScope unit="page" to="284">284</biblScope><biblScope unit="page-count">9</biblScope><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2007-08">2007-08</date></imprint><idno type="ISSN">0047-2565</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0047-2565</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Background The cell mediated immune profiles following immunization with a recombinant DNA vaccine was assessed in the simian‐human immunodeficiency virus (SHIV) and Macaque model. Earlier work demonstrated increased numbers of antigen specific CD8 and CD4 effector cells able to secrete IFN‐γ. Method The vaccine strategy included co‐immunization of a DNA based vaccine alone or in combination with a macaque IL‐12 expressing plasmid (pmacIL12). Antigen activated lymphocytes were studied for activation of a set of immunological molecules. Results The current study demonstrates lymphocytes isolated and activated from the group that was immunized with DNA and pmacIL12 had a higher level of IFN‐γ producing cells. We also observed a different immunological profile when comparing the cells isolated from macaques immunized with DNA as compared to those animals that also received pmacIL12. Conclusion The observed immune profiles are reflective of the co‐delivery of pmacIL12 and demonstrates that IL‐12 can increase the magnitude and polyfunctionality of the cellular immune response.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Maryland</li><li>Pennsylvanie</li><li>État de New York</li></region></list><tree><noCountry><name sortKey="Robinson, T M" sort="Robinson, T M" uniqKey="Robinson T" first="T. M." last="Robinson">T. M. Robinson</name></noCountry><country name="États-Unis"><region name="État de New York"><name sortKey="Sidhu, M K" sort="Sidhu, M K" uniqKey="Sidhu M" first="M. K." last="Sidhu">M. K. Sidhu</name></region><name sortKey="Boyer, J D" sort="Boyer, J D" uniqKey="Boyer J" first="J. D." last="Boyer">J. D. Boyer</name><name sortKey="Eldridge, J" sort="Eldridge, J" uniqKey="Eldridge J" first="J." last="Eldridge">J. Eldridge</name><name sortKey="Felber, B K" sort="Felber, B K" uniqKey="Felber B" first="B. K." last="Felber">B. K. Felber</name><name sortKey="Lewis, M G" sort="Lewis, M G" uniqKey="Lewis M" first="M. G." last="Lewis">M. G. Lewis</name><name sortKey="Pavlakis, G N" sort="Pavlakis, G N" uniqKey="Pavlakis G" first="G. N." last="Pavlakis">G. N. Pavlakis</name><name sortKey="Silvera, P" sort="Silvera, P" uniqKey="Silvera P" first="P." last="Silvera">P. Silvera</name><name sortKey="Weiner, D B" sort="Weiner, D B" uniqKey="Weiner D" first="D. B." last="Weiner">D. B. Weiner</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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